Research
It has been postulated that the starvation conditions encountered
during stationary phase cause an increase in the mutation frequency of E.
coli through a variety of means including decreased fidelity of DNA
replication and a reduction of in repair activity. Numerous studies have
shown that the stationary phase sigma factor has a central role in a
regulatory cascade that followed by a shift from favorable to unfavorable
growth conditions, such as when growth is restricted due to nutrient
limitation. Moreover, the stringent response regulatory nucleotides (p)ppGpp
synthesized by relA gene appear to regulate the rpoS
expression positively in E. coli. To elucidate the patterns
of stress response in E. coli, we will specifically look at recABCD,
mutHSL, rpoS, luxS, relA, spoT, and
rrn operons by using DNA arrays. |
